Composite

Part:BBa_K3257120:Design

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-10-16)


T7 Promoter-lox5171-R-PPT-RBS-EGFP-PBS-U5-R-loxP-T7 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1167
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 854
    Illegal BsaI.rc site found at 1005
    Illegal BsaI.rc site found at 1026


Design Notes

The incompatible lox sites only enable Cre recombinase to interact with the same ones respectively instead of improper cleavage happening between them. The primer binding site can be different when there is a different gene of interest needed to be reverse transcribed.


Source

Composite from the registry.

References